Coverart for item
The Resource Proteomic profiling : methods and protocols, edited by Anton Posch

Proteomic profiling : methods and protocols, edited by Anton Posch

Label
Proteomic profiling : methods and protocols
Title
Proteomic profiling
Title remainder
methods and protocols
Statement of responsibility
edited by Anton Posch
Contributor
Subject
Language
eng
Summary
This volume presents the latest developments of the main pillars of protein analysis, such as sample preparation, separation, and characterization. The book begins by describing basic but important sample preparation protocols. It then goes on to describe more sophisticated procedures on enriching specific protein classes, and concludes with detailed descriptions of integrated work-flows for comprehensive protein analysis and characterization. The authors of the individual chapters are renowned protein biochemists who have all set value to provide a detailed representation of their lab work. Throughout the chapters, these authors share important tips and tricks for a successful and reproducible employment of their protocols in other laboratories. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Proteomic Profiling: Methods and Protocols is the perfect guide for students of Biochemistry, Biomedicine, Biology, and Genomics and will be an invaluable source for the experienced, practicing scientists
Member of
Dewey number
572/.6
Illustrations
illustrations
Index
index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
Posch, Anton,
Series statement
Methods in molecular biology,
Series volume
1295
http://library.link/vocab/subjectName
  • Proteomics
  • Proteomics
Label
Proteomic profiling : methods and protocols, edited by Anton Posch
Instantiates
Publication
Copyright
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Color
multicolored
Contents
  • Simon Göddeke, Jorg Kotzka, and Stefan Lehr
  • Methods for proteomics-based analysis of the human muscle secretome using an in vitro exercise model
  • Mika Scheler [and others]
  • Urinary pellet sample preparation for shotgun proteomic analysis of microbial infection and host-pathogen interactions
  • Yanbao Yu and Rembert Pieper
  • Protocol for the parallel isolation of intact mitochondria from rat liver, kidney, heart, and brain
  • Sabine Schulz [and others]
  • Isolation of mitochondria from cultured cells and liver tissue biopsies for molecular and biochemical analyses
  • Sabine Schmitt [and others]
  • Dynamic range compression with ProteoMiner : principles and examples
  • Lei Li
  • Qualitative and quantitative proteomic analysis of formalin-fixed paraffin-embedded (FFPE) tissue
  • Omid Azimzadeh, Michael J. Atkinson, and Soile Tapio
  • Full-length protein extraction protocols and gel-based downstream applications in formalin-fixed tissue proteomics
  • Alessandro Tanca, Sergio Uzzau, and Maria Filippa Addis
  • Enrichment of low-abundant protein targets by immunoprecipitation upstream of mass spectrometry
  • Barbara Kaboord [and others]
  • Principles of protein labeling techniques
  • Christian Obermaier, Anja Griebel, and Reiner Westermeier
  • Isolation of extracellular vesicles for proteomic profiling
  • Mechanical/physical methods of cell distribution and tissue homogenization
  • Dong-Sic Choi and Yong Song Gho
  • Protocol for exosome isolation and characterization : evaluation of ultracentrifugation, density-gradient separation, and immunoaffinity capture methods
  • David W. Greening [and others]
  • Chloroplast isolation and affinity chromatography for enrichment of low-abundant proteins in complex proteomes
  • Roman G. Bayer, Simon Stael, and Markus Teige
  • Depletion of RuBisCO protein using the protamine sulfate precipitation method
  • Ravi Gupta and Sun Tae Kim
  • Step-by-step preparation of proteins for mass spectrometric analysis
  • Thomas Franz and Xinping Li
  • Identification of protein N-Termini using TMPP or dimethyl labeling and mass spectrometry
  • Stanley Goldberg
  • Jingjing Deng [and others]
  • Optimization of cell lysis and protein digestion protocols for protein analysis by LC-MS/MS
  • Dominic Winter, Alireza Dehghani, and Hanno Steen
  • Comprehensive protocol to simultaneously study protein phosphorylation, acetylation, and n-linked sialylated glycosylation
  • Marcella Nunes Melo-Braga [and others]
  • Protein profiling and phosphoprotein analysis by isoelectric focusing
  • Giuseppina Maccarrone and Michaela D. Filiou
  • Principles and examples of gel-based approaches for phosphoprotein analysis
  • Birgit Steinberger and Corina Mayrhofer
  • Neutral phosphate-affinity SDS-PAGE system for profiling of protein phosphorylation
  • Sample preservation through heat stabilization of proteins : principles and examples
  • Emiko Kinoshita-Kikuta, Eiji Kinoshita, and Tohru Koike
  • Enrichment and identification of bacterial glycopeptides by mass spectrometry
  • Nichollas E. Scott and Stuart J. Cordwell
  • In-gel peptide IEF sample preparation for LC/MS analysis
  • Tom Berkelman, Sricharan Bandhakavi, and Aran Paulus
  • Western blotting using in-gel protein labeling as a normalization control : stain-free technology
  • Jennifer E. Gilda and Aldrin V. Gomes
  • 2-D western blotting for evaluation of antibodies developed for detection of host cell protein
  • Tom Berkelman, Adriana Harbers, and Sricharan Bandhakavi
  • Free llow electrophoresis for separation of native membrane protein complexes
  • Mats Borén
  • Lutz Andreas Eichacker [and others]
  • Three-dimensional electrophoresis for quantitative profiling of complex proteomes
  • Sergio Mauro [and others]
  • Bead-based multiplex sandwich immunoassay to assess the abundance and posttranslational modification state of [beta]-catenin
  • Nicola Groll [and others]
  • Identification of SUMO E3 ligase-specific substrates using the huprot human proteome microarray
  • Eric Cox [and others]
  • Amyloid-binding proteins : affinity-based separation, proteomic identification, and optical biosensor validation
  • Alexei Medvedev [and others]
  • Proteomic profiling by nanomaterials-based matrix- assisted laser desorption/ionization mass spectrometry for high-resolution data and novel protein information directly from biological samples
  • Isolating peripheral lymphocytes by density gradient centrifugation and magnetic cell sorting
  • Suresh Kumar Kailasa and Hui-Fen Wu
  • Frederic Brosseron, Katrin Marcus, and Caroline May
  • Investigating the adipose tissue secretome : a protocol to generate high-quality samples appropriate for comprehensive proteomic profiling
Control code
ocn905871611
Dimensions
unknown
Extent
1 online resource (xv, 501 pages)
File format
unknown
Form of item
online
Isbn
9781493925490
Level of compression
unknown
Note
SpringerLink
Other control number
10.1007/978-1-4939-2550-6
Other physical details
illustrations (some color)
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)905871611
Label
Proteomic profiling : methods and protocols, edited by Anton Posch
Publication
Copyright
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Color
multicolored
Contents
  • Simon Göddeke, Jorg Kotzka, and Stefan Lehr
  • Methods for proteomics-based analysis of the human muscle secretome using an in vitro exercise model
  • Mika Scheler [and others]
  • Urinary pellet sample preparation for shotgun proteomic analysis of microbial infection and host-pathogen interactions
  • Yanbao Yu and Rembert Pieper
  • Protocol for the parallel isolation of intact mitochondria from rat liver, kidney, heart, and brain
  • Sabine Schulz [and others]
  • Isolation of mitochondria from cultured cells and liver tissue biopsies for molecular and biochemical analyses
  • Sabine Schmitt [and others]
  • Dynamic range compression with ProteoMiner : principles and examples
  • Lei Li
  • Qualitative and quantitative proteomic analysis of formalin-fixed paraffin-embedded (FFPE) tissue
  • Omid Azimzadeh, Michael J. Atkinson, and Soile Tapio
  • Full-length protein extraction protocols and gel-based downstream applications in formalin-fixed tissue proteomics
  • Alessandro Tanca, Sergio Uzzau, and Maria Filippa Addis
  • Enrichment of low-abundant protein targets by immunoprecipitation upstream of mass spectrometry
  • Barbara Kaboord [and others]
  • Principles of protein labeling techniques
  • Christian Obermaier, Anja Griebel, and Reiner Westermeier
  • Isolation of extracellular vesicles for proteomic profiling
  • Mechanical/physical methods of cell distribution and tissue homogenization
  • Dong-Sic Choi and Yong Song Gho
  • Protocol for exosome isolation and characterization : evaluation of ultracentrifugation, density-gradient separation, and immunoaffinity capture methods
  • David W. Greening [and others]
  • Chloroplast isolation and affinity chromatography for enrichment of low-abundant proteins in complex proteomes
  • Roman G. Bayer, Simon Stael, and Markus Teige
  • Depletion of RuBisCO protein using the protamine sulfate precipitation method
  • Ravi Gupta and Sun Tae Kim
  • Step-by-step preparation of proteins for mass spectrometric analysis
  • Thomas Franz and Xinping Li
  • Identification of protein N-Termini using TMPP or dimethyl labeling and mass spectrometry
  • Stanley Goldberg
  • Jingjing Deng [and others]
  • Optimization of cell lysis and protein digestion protocols for protein analysis by LC-MS/MS
  • Dominic Winter, Alireza Dehghani, and Hanno Steen
  • Comprehensive protocol to simultaneously study protein phosphorylation, acetylation, and n-linked sialylated glycosylation
  • Marcella Nunes Melo-Braga [and others]
  • Protein profiling and phosphoprotein analysis by isoelectric focusing
  • Giuseppina Maccarrone and Michaela D. Filiou
  • Principles and examples of gel-based approaches for phosphoprotein analysis
  • Birgit Steinberger and Corina Mayrhofer
  • Neutral phosphate-affinity SDS-PAGE system for profiling of protein phosphorylation
  • Sample preservation through heat stabilization of proteins : principles and examples
  • Emiko Kinoshita-Kikuta, Eiji Kinoshita, and Tohru Koike
  • Enrichment and identification of bacterial glycopeptides by mass spectrometry
  • Nichollas E. Scott and Stuart J. Cordwell
  • In-gel peptide IEF sample preparation for LC/MS analysis
  • Tom Berkelman, Sricharan Bandhakavi, and Aran Paulus
  • Western blotting using in-gel protein labeling as a normalization control : stain-free technology
  • Jennifer E. Gilda and Aldrin V. Gomes
  • 2-D western blotting for evaluation of antibodies developed for detection of host cell protein
  • Tom Berkelman, Adriana Harbers, and Sricharan Bandhakavi
  • Free llow electrophoresis for separation of native membrane protein complexes
  • Mats Borén
  • Lutz Andreas Eichacker [and others]
  • Three-dimensional electrophoresis for quantitative profiling of complex proteomes
  • Sergio Mauro [and others]
  • Bead-based multiplex sandwich immunoassay to assess the abundance and posttranslational modification state of [beta]-catenin
  • Nicola Groll [and others]
  • Identification of SUMO E3 ligase-specific substrates using the huprot human proteome microarray
  • Eric Cox [and others]
  • Amyloid-binding proteins : affinity-based separation, proteomic identification, and optical biosensor validation
  • Alexei Medvedev [and others]
  • Proteomic profiling by nanomaterials-based matrix- assisted laser desorption/ionization mass spectrometry for high-resolution data and novel protein information directly from biological samples
  • Isolating peripheral lymphocytes by density gradient centrifugation and magnetic cell sorting
  • Suresh Kumar Kailasa and Hui-Fen Wu
  • Frederic Brosseron, Katrin Marcus, and Caroline May
  • Investigating the adipose tissue secretome : a protocol to generate high-quality samples appropriate for comprehensive proteomic profiling
Control code
ocn905871611
Dimensions
unknown
Extent
1 online resource (xv, 501 pages)
File format
unknown
Form of item
online
Isbn
9781493925490
Level of compression
unknown
Note
SpringerLink
Other control number
10.1007/978-1-4939-2550-6
Other physical details
illustrations (some color)
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)905871611

Library Locations

    • InternetBorrow it
      Albany, Auckland, 0632, NZ
Processing Feedback ...