The Resource Biofilm formation by Clostridia in a dairy system : a project report presented in partial fulfilment of the requirements of the degree of Bachelor of Food Technology at Massey University, Chua Hui Yi

Biofilm formation by Clostridia in a dairy system : a project report presented in partial fulfilment of the requirements of the degree of Bachelor of Food Technology at Massey University, Chua Hui Yi

Label
Biofilm formation by Clostridia in a dairy system : a project report presented in partial fulfilment of the requirements of the degree of Bachelor of Food Technology at Massey University
Title
Biofilm formation by Clostridia in a dairy system
Title remainder
a project report presented in partial fulfilment of the requirements of the degree of Bachelor of Food Technology at Massey University
Statement of responsibility
Chua Hui Yi
Creator
Contributor
Author
Degree granting institution
Subject
Language
eng
Summary
The aim of this project is to determine the capability of Clostridia to form biofilms in a dairy system. In light of the worldwide whey protein concentrate recall in 2013 caused by Clostridium sporogenes, five strains of C. sporogenes were harvested for spores and screened for biofilm formers through microtitre plate assays in reinforced clostridial medium (RCM) and reconstituted skimmed milk (RSM) in MOPS buffer. They were incubated up to 5 days and optical densities were checked at 570 nm each day. The strongest biofilm former was selected for trials in rotating disc reactors using stainless steel coupons as they simulate a flowing milk system closely to industrial settings. It was found that 3 out of 5 strains were forming good biofilms in RCM in microtitre plate assays when the data was statistically compared to control. Weaker biofilms were observed in RSM as the conditions were not anaerobic enough. The capability of strain 1097 forming biofilm was confirmed as high counts were found for RCM denoted as "TNTC", and slightly less for RSM from reactor trials. This suggests that when the environment is anaerobic enough, biofilm formation at 37°C by Clostridia is possible in a simulated dairy system. It is recommended to perform higher dilutions for plate counting after reactor trials in order to obtain more accurate counts of the biofilm. Additionally, confocal laser scanning microscopy can be used to determine the thickness of biofilm--Summary
Cataloging source
UW1
http://library.link/vocab/creatorName
Chua, Hui Yi
Degree
BFoodTech(Hons)
Dissertation year
2014
Granting institution
Massey University
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • abstracts summaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
  • Massey University
  • Massey University
http://library.link/vocab/subjectName
  • Dairy microbiology
  • Clostridium
  • Clostridium sporogenes
  • Biofilms
Target audience
specialized
Label
Biofilm formation by Clostridia in a dairy system : a project report presented in partial fulfilment of the requirements of the degree of Bachelor of Food Technology at Massey University, Chua Hui Yi
Instantiates
Production
Note
  • At head of title: Massey University, Singapore
  • At head of cover title: Massey University. Institute of Food, Nutrition and Human Health final year project report
Bibliography note
Includes bibliographical references
Carrier category
volume
Carrier MARC source
rdacarrier
Content category
text
Content type MARC source
rdacontent
Contents
Introduction -- Literature review. Definition of biofilms ; Clostridium species -- Materials and methods. Spore harvesting ; Spore enumeration ; Mictotitre plate assays ; Statistical analysis ; Rotating disc reactor -- Results and discussion. Spore enumeration ; Microtitre plate assay ; Statistical assays of Microtitre plate assays ; Rotating disc reactor trials ; Plate counts of biofilm from the reactor trials -- Conclusions ; Recommendations
Dimensions
30 cm
Extent
v, 44 pages
Media category
unmediated
Media MARC source
rdamedia
Other physical details
illustrations
Label
Biofilm formation by Clostridia in a dairy system : a project report presented in partial fulfilment of the requirements of the degree of Bachelor of Food Technology at Massey University, Chua Hui Yi
Production
Note
  • At head of title: Massey University, Singapore
  • At head of cover title: Massey University. Institute of Food, Nutrition and Human Health final year project report
Bibliography note
Includes bibliographical references
Carrier category
volume
Carrier MARC source
rdacarrier
Content category
text
Content type MARC source
rdacontent
Contents
Introduction -- Literature review. Definition of biofilms ; Clostridium species -- Materials and methods. Spore harvesting ; Spore enumeration ; Mictotitre plate assays ; Statistical analysis ; Rotating disc reactor -- Results and discussion. Spore enumeration ; Microtitre plate assay ; Statistical assays of Microtitre plate assays ; Rotating disc reactor trials ; Plate counts of biofilm from the reactor trials -- Conclusions ; Recommendations
Dimensions
30 cm
Extent
v, 44 pages
Media category
unmediated
Media MARC source
rdamedia
Other physical details
illustrations

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